Hi, Anita If you could find a way to test the elute's activity/binding to its' substrat/cofactor, then you will learn much more about your target. If the function assay is elusive, you could try superose column (5KDa-5MKDa). Does your light scattering tell you about the estimated size and MW?
Best, Joe On Sat, Aug 27, 2011 at 1:29 AM, anita p <crystals...@gmail.com> wrote: > Hi Yury, > I have done dynamic light scattering and it shows its polydispersed. > Please let me know if it is still ok for setting trays. > reg. > anita > > On Fri, Aug 26, 2011 at 10:21 PM, Yuriy Patskovsky < > yuriy.patskov...@einstein.yu.edu> wrote: > >> Anita, >> an assembly may be quite large - I would check it somehow, maybe by light >> scattering or centrifugation >> >> Good luck >> >> Yury >> ------------------------------ >> *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of anita p >> [crystals...@gmail.com] >> *Sent:* Friday, August 26, 2011 3:03 AM >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* [ccp4bb] Protein aggregation and crystallization >> >> Hi All, >> I am working on a protein which has a membrane spanning region and as >> cytosolic domain.I have made various deletion constructs of the protein, so >> that I can have a crystallizable fragment. There is no homologues mentioned >> in the pdb for this protein. >> All of these constructs are purified successfully but when concentrated >> and loaded on a gel filtration column Superdex-200, they elute in the void >> volume. But the proteins donot precipitate out.... !! >> Is it worth while to go ahead for crystallization trials?? >> Any other suggestion is most welcome. >> Thanks >> Anita >> >> >
