Hi Yury, I have done dynamic light scattering and it shows its polydispersed. Please let me know if it is still ok for setting trays. reg. anita
On Fri, Aug 26, 2011 at 10:21 PM, Yuriy Patskovsky < yuriy.patskov...@einstein.yu.edu> wrote: > Anita, > an assembly may be quite large - I would check it somehow, maybe by light > scattering or centrifugation > > Good luck > > Yury > ------------------------------ > *From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of anita p [ > crystals...@gmail.com] > *Sent:* Friday, August 26, 2011 3:03 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Protein aggregation and crystallization > > Hi All, > I am working on a protein which has a membrane spanning region and as > cytosolic domain.I have made various deletion constructs of the protein, so > that I can have a crystallizable fragment. There is no homologues mentioned > in the pdb for this protein. > All of these constructs are purified successfully but when concentrated and > loaded on a gel filtration column Superdex-200, they elute in the void > volume. But the proteins donot precipitate out.... !! > Is it worth while to go ahead for crystallization trials?? > Any other suggestion is most welcome. > Thanks > Anita > >
