Hi All,
I am working on a protein which has a membrane spanning region and as
cytosolic domain.I have made various deletion constructs of the protein, so
that I can have a crystallizable fragment. There is no homologues mentioned
in the pdb for this protein.
All of these constructs are purified successfully but when concentrated and
loaded on a gel filtration column Superdex-200, they elute in the void
volume. But the proteins donot precipitate out.... !!
Is it worth while to go ahead for crystallization trials??
Any other suggestion is most welcome.
Thanks
Anita
