Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate cryo

[Chris Ulens]

Hi,
I would like to get recommendations for a proper cryo solution for a  
crystallization hit from the Hampton crystal screen Ammonium di- 
hydrogen phosphate 0.4M. I tried increasing glycerol up to 30% with  
the same ammonium phosphate concentration or increasing glycerol up to  
30% in the presence of 1.3M ammonium phosphate. Both gave iced up  
drops (I only tried quick and dirty tests by dipping a cover glass in  
liquid nitrogen).

Thank you.
-Chris