Dear Chris,
Indeed,. according to McFerrin and Snell, 2002, Appl. Crystallogr.,
they recommend 30%(v/v) PEG400, or 35% EG (ethlylene glycol) or 30%
PG (propylene glycol)
However, they also mention the use of 35% (v/v) glycerol.
regards
Kristof
On 26 May 2011, at 13:01, herman.schreu...@sanofi-aventis.com wrote:
Dear Chris,
I have not used this particular condition, but as a rule of thumb I
use
"like with like", e.g. glycerol, ethylene glycol, PEG400 etc. with PEG
conditions, and saltlike compounds (sucrose, xylitol, salts) for salt
conditions. In your case I would try glucose or xylitol or just look
what happens if you increase the ammonium phosphate concentration
to say
2M without adding glycerol.
Good luck!
Herman
-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Chris Ulens
Sent: Thursday, May 26, 2011 12:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate
cryo
Hi,
I would like to get recommendations for a proper cryo solution for a
crystallization hit from the Hampton crystal screen Ammonium di-
hydrogen phosphate 0.4M. I tried increasing glycerol up to 30% with
the
same ammonium phosphate concentration or increasing glycerol up to 30%
in the presence of 1.3M ammonium phosphate. Both gave iced up drops (I
only tried quick and dirty tests by dipping a cover glass in liquid
nitrogen).
Thank you.
-Chris
--------------------------------------
Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
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