Re: [ccp4bb] TEV protease

[Badreddine]

I daily use Tev protease for a ration 1:20 (Tev-protein) but the optimal 
activity of TEV is at 31°C for 1 hour, if there is not the case, you can 
put the clivage reaction at 4°C for ON.
In some cases, cleavage is not at 100% (SDS-PAGE control) and you need 
to put reaction for more time (2 days approximately).
 In lectures, it is described that they use a differents ratio (1:5, 
1:10, 1:20) but i think the efficient way is to test differents ratio 
with a small volume reaction (20uL for example).

B.
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