[ccp4bb] TEV protease

[Gloria Borgstahl]

We are using TEV protease to make a big batch of protein for
structural studies.  We usually use thrombin, so this is our first
time using this enzyme on a large scale.  Boy is it expensive!  Does
anyone know of a bulk source for this enzyme and what ratios of use do
you recommend.  Imidazole inhibits?
Many thanks. Gloria