Hi All

Both Martin and Kay ( in a later message) have misinterpreted what I wrote - what I meant was that it seems normal in using XDS with Pilatus data, not the normal thing to do with data from other detectors.

I had found a number of scripts on the web that deal specifically with processing Pilatus data with XDS that seemed to use "PATCH_SHUTTER_PROBLEM=TRUE" - what I didn't notice was that the commands had all been commented out, and that "FALSE", the default, was in operation.

So, in either case I was wrong. Apologies to Kay and Wolfgang, and all who write and use scripts for processing Pilatus data with XDS.

On 5 Nov 2010, at 18:33, Martin Hallberg wrote:

Hi Harry,

On Nov 5, 2010, at 5:45 PM, Harry Powell wrote:

I think there may be issues with collecting data too finely with a Pilatus, even in shutterless mode. I don't know where the problems arise (can't be shutter/rotation axis synchronisation), but it seems to be the normal thing in XDS (which should have no problems with fine phi-slicing) to use the "PATCH_SHUTTER_PROBLEM=TRUE" that Martin Hallberg suggested, which looks a bit like a fudge to me (but I expect Kay to correct me on that!).

It is not the normal thing in XDS but it is perhaps a relatively common solution to shutter/spindle synchronisation problems discovered afterwards (always process directly at the beam line!). The default in XDS is indeed PATCH_SHUTTER_PROBLEM=FALSE

Compensating like this is of course not the best (go and recollect!) but still way better than unusable data in the meantime. In the case Sergei originally described it would at least indicate what the problem may be. Sergei did not say which detector was used for the data collection so we don't know if it was a Pilatus or a CCD. Maybe Sergei can fill us in on the details?

BTW, any views in the community on crystal lifetime with continuous data collection like on the Pilatus (or AXIOM) versus letting the crystal rest/cool/die(?) a second between frames on a CCD?

Cheers,

Martin






Harry
--
Dr Harry Powell,
MRC Laboratory of Molecular Biology,
Hills Road,
Cambridge,
CB2 0QH

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