A2220 Sigma ANTI-FLAG® M2 Affinity Gel
On Nov 2, 2010, at 11:01 AM, Oganesyan, Vaheh wrote: > On the same note with Mirek: does anyone know of a source other than GE for > resin for purification of FLAG-ed proteins? > > Thanks. > > Vaheh > > -----Original Message----- > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Mirek > Cygler > Sent: Tuesday, November 02, 2010 11:47 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Glutathione sepharose > > Hello, > For various reasons we are frequently expressing proteins with a GST > tag. The glutathione sepharose beads that we are using for affinity > purification seem to be difficult to regenerate and we see much lower > capacity when used the second time. We are following the manufacturer's > instructions for regeneration but this not very effective process as opposed > to NiNTA which can regenerate multiple times. Due to this, the purification > of GST-tagged proteins is quite costly. Do you know of a GSH-sepharose that > can be successfully regenerated several times? Any comments will be greatly > appreciated. > > Mirek > > > > To the extent this electronic communication or any of its attachments contain > information that is not in the public domain, such information is considered > by MedImmune to be confidential and proprietary. This communication is > expected to be read and/or used only by the individual(s) for whom it is > intended. If you have received this electronic communication in error, > please reply to the sender advising of the error in transmission and delete > the original message and any accompanying documents from your system > immediately, without copying, reviewing or otherwise using them for any > purpose. Thank you for your cooperation.
