When diffraction fails - one can always look at the crystals with a UV microscope (assuming you have Trp) - they should light up. Or if its possible - harvest them, wash and run a gel. (or if its detergent a TLC)
Joe 2010/9/1 wu donghui <wdh0...@gmail.com> > It does not look like protein crystal and IZIT staining is not reliable in > determining protein or other. Mostly it is like detergent or PEG crystal or > quasi-crystal. > > Good luck > > Wu > > 2010/8/31 qiangm zhang <zhangqia...@gmail.com> > > Hi all, >> >> I got a crystal of one membrane protein (~60kD) from Na/K phosphate >> condition (see getit_4), then I got the improved crystal like getit_5 after >> trying seeding, different detergents, lipids and additives. But this crystal >> does not diffract at all, I already tried Izit staining which shows it is >> protein crystal (detergent crystal?). Does anyone have any good suggestions >> for the optimization of this membrane protein crystallization? Thank you in >> advance. >> >> Best regards >> >> Qiangmin Zhang >> >> Biomedical Science Tower 3 >> Room1034 >> 3501 Fifth Avenue >> Pittsburgh, PA 15260 >> >> >> >> >> >> -- >> 张强敏 >> > >
