Hello Qiangmin, Here are links to a few web resources that have tips for membrane protein crystallization that may help with your optimization strategy:
http://kb.psi-structuralgenomics.org/update/2009/06/full/th_psisgkb.2009.25.html http://web.emeraldbiosystems.com/blog/bid/33916/8-Practical-Tips-for-Membrane-Protein-Crystallization http://mcl1.ncifcrf.gov/nihxray/Tips-and-Tricks_Crystallization_Membrane_Protein.pdf good luck, Eric __________________________ Eric Larson, PhD Biomolecular Structure Center Department of Biochemistry Box 357742 University of Washington Seattle, WA 98195 On Mon, 30 Aug 2010, qiangm zhang wrote:
Hi all, I got a crystal of one membrane protein (~60kD) from Na/K phosphate condition (see getit_4), then I got the improved crystal like getit_5 after trying seeding, different detergents, lipids and additives. But this crystal does not diffract at all, I already tried Izit staining which shows it is protein crystal (detergent crystal?). Does anyone have any good suggestions for the optimization of this membrane protein crystallization? Thank you in advance. Best regards Qiangmin Zhang Biomedical Science Tower 3 Room1034 3501 Fifth Avenue Pittsburgh, PA 15260 -- 张强敏
