It does not look like protein crystal and IZIT staining is not reliable in determining protein or other. Mostly it is like detergent or PEG crystal or quasi-crystal.
Good luck Wu 2010/8/31 qiangm zhang <zhangqia...@gmail.com> > Hi all, > > I got a crystal of one membrane protein (~60kD) from Na/K phosphate > condition (see getit_4), then I got the improved crystal like getit_5 after > trying seeding, different detergents, lipids and additives. But this crystal > does not diffract at all, I already tried Izit staining which shows it is > protein crystal (detergent crystal?). Does anyone have any good suggestions > for the optimization of this membrane protein crystallization? Thank you in > advance. > > Best regards > > Qiangmin Zhang > > Biomedical Science Tower 3 > Room1034 > 3501 Fifth Avenue > Pittsburgh, PA 15260 > > > > > > -- > 张强敏 >
