You should mass spec your samples to determine what has crystallized, possible
e coli contaminant.
M. Gordon Joyce,
Visiting Fellow,
Structural Immunology Section,
Laboratory of Immunogenetics,
NIH/NIAID,
12441 Parklawn Drive,
Rockville,
MD 20851
Phone: 301 594 0242 Office
301 496 3792 Lab
-----Original Message-----
From: Jon Schuermann [mailto:schue...@anl.gov]
Sent: Thursday, February 18, 2010 4:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Expression of a protein of 43KDa
Maybe I am not getting it, but I don't see your problem or question?
What I understand from your message is that you have a protein that
overexpresses, purifies, and crystallizes... where's the problem? We all wish
we had that problem...
Jon
On 02/18/2010 11:49 AM, Armando Albert de la Cruz wrote:
> I am trying to overexpress a His-tagged protein of 29KDa in E.coli
> (BL21-codon plus) and I end up with a highly expressed product that of
> 43KDa that binds to the Ni-column. I also have nice crystals. Does
> anyone have any experience on this.
> Armando
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
Argonne, IL 60439
email: schue...@anl.gov
Tel: (630) 252-0682
Fax: (630) 252-0687
