Maybe I am not getting it, but I don't see your problem or question?
What I understand from your message is that you have a protein that
overexpresses, purifies, and crystallizes... where's the problem? We all
wish we had that problem...
Jon
On 02/18/2010 11:49 AM, Armando Albert de la Cruz wrote:
I am trying to overexpress a His-tagged protein of 29KDa in E.coli
(BL21-codon plus) and I end up with a highly expressed product that of
43KDa that binds to the Ni-column. I also have nice crystals. Does
anyone have any experience on this.
Armando
--
Jonathan P. Schuermann, Ph. D.
Beamline Scientist
NE-CAT, Building 436E
Advanced Photon Source (APS)
Argonne National Laboratory
9700 South Cass Avenue
Argonne, IL 60439
email: schue...@anl.gov
Tel: (630) 252-0682
Fax: (630) 252-0687
