EF-Tu Poul On 18/02/2010, at 18.49, Armando Albert de la Cruz wrote:
I am trying to overexpress a His-tagged protein of 29KDa in E.coli (BL21-codon plus) and I end up with a highly expressed product that of 43KDa that binds to the Ni-column. I also have nice crystals. Does anyone have any experience on this.Armando
