Dear Steve, I would also use Damian's approach, but the sequence of the core should be NAG-NAG-BMA-(MAN)2. This is improtant because the correct stereochemistry restraints for beta-mannose can only be applied when you call the residue BMA. Building carbohydrates also comes with special validation requirements. PDB-care and CARP are both very usefull. Unfortunately, the service is currently down (http://www.dkfz.de/spec/glycosciences.de). Just make sure the links between your carbs are correct and, please, remove the O1 atoms when needed ;)
Cheers, Robbie Joosten ---------------------------------------- > Date: Mon, 21 Dec 2009 17:48:31 -0800 > From: dceki...@scripps.edu > Subject: Re: [ccp4bb] Coot pudding? (a.k.a N-linked carbohydrate addition) > To: CCP4BB@JISCMAIL.AC.UK > > Steve, > > My general strategy is to start with an "ideal" glycan (an Asn linked to > NAG-NAG-(MAN)3 ) and superimpose the Asn on the residue from my protein. > Then you can move the whole glycan as a rigid body until the Asn and > first NAG are roughly positioned. Then you can tweak any sugars further > out on the chain to get them to fit. Unless you have really great > density, usually it is best to avoid real pace refine zone. Better to > fit the sugars using the manual rigid body fitting tools, do the best > you can, then REFMAC usually brings them in OK. > > I have some models that I could send you if you need them. > > Best, > > Damian Ekiert > > > > Soisson, Stephen M wrote: > > Hi everyone- > > > > I was searching for some information on what might be the best way to > > add N-linked sugars in coot, and Google has let me down. Searching > > "adding sugars in coot" returns a very nice recipe for Coot Pudding. > > > > ***_Recipe for_/ Coot//__/_ Pudding - American_/ Coots/* > > ******** It has plenty of fat,/ > > sugar/, and starch, and probably some calcium from the milk.* ...* The/ > > coots/ will not tolerate/ adding/ eggs in any form, so this is an egg* ...* > > ///_www.beaky_//_*coot*.com/pudding.html_/// > > ///// -/ _Similar_ > > // > > > > > > > > I did not know that coots had such an aversion to eggs. :) > > > > Anyway, would anyone have any top tips on adding N-linked sugars using > > coot? I can import the NAG monomers, but linking them up to the protein > > seems non-trivial > > > > Many thanks in advance, > > > > Steve > > > > > > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
