We used to use another glass cover slip and make a sandwich with the drop and rub together. Recovery is a bit messy though.
Greg Snyder, Ph.D. Postdoctoral Fellow Laboratory of Immunology Structural Immunobiology Section National Institute of Allergy and Infectious Diseases National Institutes of Health Building 4, Room 138 4 Center Drive Bethesda, MD 20892-0430 U.S.A. Phone: 301-402-7523 FAX: 301-480-1291 E-mail: gsny...@niaid.nih.gov http://www3.niaid.nih.gov/labs/aboutlabs/li/StructuralImmunobiology/ The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error, please inform the sender and delete it from your mailbox or any other storage devices. The National Institute of Allergy and Infectious Diseases (NIAID) shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives. -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Ramanathan Natesh Sent: Saturday, September 12, 2009 10:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Preparation of seed-stocks without seed-beads Hi Most probably you might have micro seeds stock in your present well. A streak with cat whisker/something like that in your old well and immedietly a streak in your new well might work miracles sometimes. HTH all the best Natesh. > > >> Dear CCP4bbers, > >> > >> Can anyone suggests how to make seed-stocks if one is not having > >> seed-beads... Is there any other methods to crush the crystals for > >> the same purpose. What if it is simple vortexed. Off-course there > >> wold be all sorts of sizes, the intact crystals as well. > >> Please suggest. > >> > >> Thanks a lot more for previous help. > >> James. > > --------------------------------------- Ramanathan Natesh Researcher ID: C-4488-2008 (http://www.researcherid.com/rid/C-4488-2008) Wellcome Trust Advanced Training Fellow School of Crystallography Birkbeck College University of London Malet Street London WC1E 7HX Tel: +44 (0)20-7631-6850/6824 Fax: +44 (0)20-7631-6803 --------------------------------------- "Live Simply and do Serious Things .. " - Dorothy Mary Crowfoot Hodgkin OM, FRS "In Science truth always wins" - Max Ferdinand Perutz OM FRS ---------------------------------------
