You can melt the end of a thin glass capillary, forming a glass 'bead' at one end. You can then attack your drop, containing the crystals to made into seeds, with the beaded end of the capillary. You can then pipette up the crushed crystals, and dilute and/or spin to make your seed stock.
With regards, Tony. >> Dear CCP4bbers, >> >> Can anyone suggests how to make seed-stocks if one is not having >> seed-beads... Is there any other methods to crush the crystals for the >> same >> purpose. What if it is simple vortexed. Off-course there wold be all sorts >> of sizes, the intact crystals as well. >> Please suggest. >> >> Thanks a lot more for previous help. >> James. ---- Dr Antony W. Oliver Senior Scientist Cancer Research UK DNA Repair Enzymes Group Section of Structural Biology The Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB antony.oli...@icr.ac.uk 020 7153 5571 ---- The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network.
