Re: [ccp4bb] Preparation of seed-stocks without seed-beads

[Ezra Peisach]

We used to use a needle to crush the crystals - and then make serial 
dilutions....

ucbccka wrote:
sonication

  
Dear CCP4bbers,

Can anyone suggests how to make seed-stocks if one is not having
seed-beads... Is there any other methods to crush the crystals for the
same
purpose. What if it is simple vortexed. Off-course there wold be all sorts
of sizes, the intact crystals as well.
Please suggest.

Thanks a lot more for previous help.
James.