I second that one. Ammonium sulfate is one of my favorite cryos. I
recommend making up a saturated solution of ammonium sulfate, as it is
actually a very good cryo all by itself, and then "dilute" it by adding
the rest of the stuff in your condition (buffers, etc. and a little bit
of water). You want to be a little below saturation so that the salt
does not grow crystals of its own while you are soaking. This is
especially important if your protein crystals are hexagonal rods!
-James Holton
MAD Scientist
Savvas Savvides wrote:
Hi Brenda
Try > 3M ammonium sulfate itself! We have tried that with great succes by
cryo-cooling xtals grown at 3.2M AS straight out of their crystallization
drops. You can consult the "M&M" section in Kyndt J. et al Biochemistry 2007
Jan 9;46(1):95-105 for a more detailed description of what we did.
Best of luck
Savvas
----
Savvas Savvides
L-ProBE, Unit for Structural Biology
Ghent University
K.L. Ledeganckstraat 35
9000 Ghent, BELGIUM
office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19
Email: savvas.savvi...@ugent.be
http://www.lprobe.ugent.be/xray.html
-----Original Message-----
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Schulman, Brenda
Sent: Wednesday, August 19, 2009 6:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprotectants for crystals growing
in > 3M ammonium sulfate.
Thanks!
Brenda
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