I would also include a NaI and NaBr quick soak in 250 mM, SCN- is
considered a pseudohalide
cheers Preben
Sebastiano Pasqualato wrote:
Hi all,
I've got crystals of a protein of ca 200 residues, with 2 free
cysteines, 5 histidines, 2 methionines.
We have nice diffraction for the native crystals, that grow in 150 mM
KSCN, 17% PEG 3350, bis tris propane pH 8.8.
We are crystallising the SeMet derivative, but I'm not completely sure
I will be able to have nice crystals by saturday, when we have tunable
time at the ESRF.
I was thinking of trying with some heavy atom soaks, but only have
like 30 crystals, so limited trials allowed!
Which compound would you advice as more likely to work, and thus worth
testing?
Thanks in advance for the suggestions,
ciao
s
--Sebastiano Pasqualato, PhD
IFOM-IEO Campus
Dipartimento di Oncologia Sperimentale
Istituto Europeo di Oncologia
via Adamello, 16
20139 - Milano
Italy
tel +39 02 9437 5094
--
Jens Preben Morth, Ph.D
Aarhus University
Department of Molecular Biology
Gustav Wieds Vej 10 C
DK - 8000 Aarhus C
Tel. +45 8942 5257, Fax. +45 8612 3178
j...@mb.au.dk
website: http://person.au.dk/da/j...@mb
