Hi, 1. KSCN will suck up a lot of the Hg and other metals -- can you switch to something else? 2. Hg typically does not enter the protein core. 3. It's generally not necessary to use a special tank, as long as you treat the resulting solutions as heavy-atom waste; but of course each lab's safety precautions are different.
Artem > > Does mercury tends to get into the protein core to denature protein or > not? > > For gel-shift assay, do people normally use a special gel tank for heavy > metal work? > > thanks in advance > > Xiaoli Xiong (Alex) > > > --On 15 July 2009 14:33 +0200 Sebastiano Pasqualato > <sebastiano.pasqual...@ifom-ieo-campus.it> wrote: > >> Hi all, >> I've got crystals of a protein of ca 200 residues, with 2 free >> cysteines, 5 histidines, 2 methionines. >> We have nice diffraction for the native crystals, that grow in 150 mM >> KSCN, 17% PEG 3350, bis tris propane pH 8.8. >> We are crystallising the SeMet derivative, but I'm not completely sure >> I >> will be able to have nice crystals by Saturday, when we have tunable >> time at the ESRF. >> I was thinking of trying with some heavy atom soaks, but only have like >> 30 crystals, so limited trials allowed! >> Which compound would you advice as more likely to work, and thus worth >> testing? >> Thanks in advance for the suggestions, >> ciao >> s >> >> >> >> -- >> Sebastiano Pasqualato, PhD >> IFOM-IEO Campus >> Dipartimento di Oncologia Sperimentale >> Istituto Europeo di Oncologia >> via Adamello, 16 >> 20139 - Milano >> Italy >> >> tel +39 02 9437 5094 > > > > ---------------------- > Xiaoli Xiong > PhD Candidate > Department of Cellular and Molecular Medicine > School of Medical Sciences > University of Bristol > University Walk > Bristol BS8 1TD, UK > x.l.xi...@bristol.ac.uk >
