Hi Sebastiano, Have a look at HATODAS:
http://hatodas.harima.riken.go.jp/ Good luck, Miguel Le 15 juil. 09 à 14:33, Sebastiano Pasqualato a écrit :
Hi all,I've got crystals of a protein of ca 200 residues, with 2 free cysteines, 5 histidines, 2 methionines. We have nice diffraction for the native crystals, that grow in 150 mM KSCN, 17% PEG 3350, bis tris propane pH 8.8. We are crystallising the SeMet derivative, but I'm not completely sure I will be able to have nice crystals by saturday, when we have tunable time at the ESRF. I was thinking of trying with some heavy atom soaks, but only have like 30 crystals, so limited trials allowed! Which compound would you advice as more likely to work, and thus worth testing?Thanks in advance for the suggestions, ciao s -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094 -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
-- Miguel Ortiz Lombardía Architecture et Fonction des Macromolécules Biologiques (UMR6098) CNRS, Universités d'Aix-Marseille I & II Case 932 163 Avenue de Luminy 13288 Marseille cedex 9 France Tel : +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 e-mail: miguel.ortiz-lombar...@afmb.univ-mrs.fr Web: http://www.pangea.org/mol/spip.php?rubrique2
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