Dear Sang They are really different!
And I guess you would probably want to use NCS restraints depending on your resolution. Regards, Folmer 2009/3/24 Sang Hoon Joo <s...@duke.edu>: > I am refining my crystal structure in which I have two identical > chains in one asymmetric unit. > Space group is H32 and each chain yields me a biological trimer as expected. > The problem is, do I have to assume they are identical, or they are > really different. > After each cycle of refinement, if I try to align two molecules I get > ~ 0.17 RMSD. > -- > Sang Hoon Joo, PhD > Postdoctoral Associate > Duke University > 239 Nanaline H. Duke > Box 3711, DUMC > Durham, NC 27710 >
