I am refining my crystal structure in which I have two identical chains in one asymmetric unit. Space group is H32 and each chain yields me a biological trimer as expected. The problem is, do I have to assume they are identical, or they are really different. After each cycle of refinement, if I try to align two molecules I get ~ 0.17 RMSD. -- Sang Hoon Joo, PhD Postdoctoral Associate Duke University 239 Nanaline H. Duke Box 3711, DUMC Durham, NC 27710
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