Dear sir, Thank you all of you for your suggestions.
Sincerely Deb On Fri, 05 Dec 2008 Mathews,Irimpan wrote : >Hi Deb, > >You may want to try some ions (cations and anions). It may help stabilize some >loops if they have binding sites. I usually add around 5 - 10uL to 500 uL of >the well solution to see their effect. > >good luck, >Mathews > > >________________________________ > > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Debajyoti > Dutta >Sent: Thursday, December 04, 2008 9:04 PM >To: CCP4BB@JISCMAIL.AC.UK >Subject: Re: [ccp4bb] getting weak diffracting crystals > > > > >Hi again, > >Thank you for your reply. > >I know that the loop regions are notorious for crystallization however in this >case it is yielding the crystals but with lower quality. Does it not due to >the quick crystallization, the lattice has no time to form up. That's why it >is happening. > >Can I not opt for glycerol, or sitting drop method. > >Sincerely >Deb > > >On Thu, 04 Dec 2008 [EMAIL PROTECTED] wrote : > >Dear Deb, > > > >We've seen the detrimental effects of local disorder time and tme again. > >So - yes it is very likely that the putative disordered loops > >detrimentally affect the quality of your crystals. > > > >You can try to engineer your protein to be better - it usually takes a > >number of internally engineered constructs to get things right (about 10 > >per 350aa protein, in my experience). This is different from terminal > >truncations - which also can have a huge effect on crystallization. > > > >You might also try some of the methods summarized here: > >http://www.xtals.org/pdfs/rescue_crystals.pdf > > > > > >Cheers, > > > >Artem > > > > > > Dear Members, > > > > > > I am getting crystals of my protein. The secondary structure prediction > > > implies that it has N-terminal with high degree of loop regions. I also > > > get some mountable crystals yielding weak diffraction pattern(10 A). The > > > quality of the crystals can also be assumed from its texture for it has > > > tortuous surface. The big crystals are achieved at very high concentration > > > in hanging drop method (44mg/ml) whereas the initial hit (small crystals) > > > is at lower concentration in sitting drop (~ 5mg/ml). > > > > > > I have some queries about it. Does the N -terminal loop regions are having > > > any effect of the crystal quality. Any suggestions of its quality > > > improvement are welcome. I will be highly benefited with your generous > > > replies. > > > > > > Sincerely > > > Deb > > > > > > > > >
