Dear Members, I am getting crystals of my protein. The secondary structure prediction implies that it has N-terminal with high degree of loop regions. I also get some mountable crystals yielding weak diffraction pattern(10 A). The quality of the crystals can also be assumed from its texture for it has tortuous surface. The big crystals are achieved at very high concentration in hanging drop method (44mg/ml) whereas the initial hit (small crystals) is at lower concentration in sitting drop (~ 5mg/ml).
I have some queries about it. Does the N terminal loop regions are having any effect of the crystal quality. Any suggestions of its quality improvement are welcome. I will be highly benefited with your generous replies. Sincerely Deb
