Wudong--
What is your ratio of protein to ligand? You might want to try 1:3, 1:5
or 1:10 ratios of protein : ligand. This can make a HUGE difference.
There are instances where the organic solvents (DMSO, EtOH) used to
dissolve the ligands interfere with the crystallization process. In this
instance I tried the following:
put dry cmpd in the crystallization drop with the protein (does not
matter if it does not totally dissolve)
added the dry cmpd to the protein solution & put on a LabQuake shaker
overnight with gentle rotation. Centrifuge the next morning & set up your
crystallization screens.
Good Luck!
annie
Annie Hassell
Glaxo Smithkline
5 Moore Drive
RTP, NC 27709
919/483-3228
919/483-0368 (FAX)
[EMAIL PROTECTED]
"charliswu" <[EMAIL PROTECTED]>
Sent by: "CCP4 bulletin board" <CCP4BB@JISCMAIL.AC.UK>
10-Oct-2008 23:17
Please respond to "charliswu" <[EMAIL PROTECTED]>
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
[ccp4bb] Substrate(water insoluble) Soaking
Hi All,
I am now soaking two substrates to the crystal.But one is soluble in
ethanol and one is soluble in DMSO. After co-crystallization and soaking,
no substrate found in the structure.
Does anybody has the experience in dealing this problem?
Any comments or suggestions would be highly welcome.
WuDong
IBP Beijing CAS
[EMAIL PROTECTED]
