Hi there... http://www.ncbi.nlm.nih.gov/pubmed/17164529 Hassell, AM et al, (2007) Crystallisation of Protein-ligand complexes Acta Cryst D63,p72
This paper is an excellent review of "how to get my ligand into my crystal" and includes some methods for dealing with insoluble ligands. HTH, David 2008/10/11 charliswu <[EMAIL PROTECTED]>: > > > > Hi All, > I am now soaking two substrates to the crystal.But one is soluble in > ethanol and one is soluble in DMSO. After co-crystallization and soaking, no > substrate found in the structure. > Does anybody has the experience in dealing this problem? > Any comments or suggestions would be highly welcome. > WuDong > IBP Beijing CAS > [EMAIL PROTECTED] -- ============================ David C. Briggs PhD Father & Crystallographer http://drdavidcbriggs.googlepages.com/home AIM ID: dbassophile ============================
