The Bio-Rad Flamingo Fluorescent Gel Stain has a very linear profile
with protein concentration (if you believe the standard curves in the
manual). In my hands it gives nice results for binding assays.
Cheers,
-Greg Alushin
On Sep 25, 2008, at 2:35 PM, Jacob Keller wrote:
Dear Crystallographers,
I remember having seen on this listserve that coomassie stain is
horribly non-linear in intensity per protein concentration, which
leads me to two questions:
1. Does anybody have a reference for quantitation of coomassie's
linearity (and possibly other stains), i.e. where and how extensive
the linear range is, and
2. Can anybody suggest a more linear protein gel stain?
Thanks,
Jacob Keller
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
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