On 25 Sep 2008, at 14:35, Jacob Keller wrote:
Dear Crystallographers,
I remember having seen on this listserve that coomassie stain is
horribly non-linear in intensity per protein concentration, which
leads me to two questions:
1. Does anybody have a reference for quantitation of coomassie's
linearity (and possibly other stains), i.e. where and how extensive
the linear range is, and
2. Can anybody suggest a more linear protein gel stain?
Silver stain would be an option. Not quite sure how the linearity is
when using fluorescent probes e.g. SyproOrange for protein staining.
Jürgen
Thanks,
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
*******************************************
-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
