with regard to what is "guaranteed"--usually you get a mass spec /
HPLC etc
sheets telling you what the real purity is. checking that is certainly
worthwhile.
i would perhaps first try with 95% pure and then if not good enough
purify further.
this purity scale has worked ok for us.
tommi
On Aug 28, 2008, at 4:29 PM, Remy Loris wrote:
Dear Daniel,
We used peptides (up to 40 aa long) from BioSynthesis, Lewisville,
Texas (www.biosyn.com) at 90% purity with great success. The
material is guaranteed to be 90% (or whatever you ask) pure but in
reality it is a lot better. (I have no commerical interest here
myself). In one case we even crystallized a complex with peptide
that was only supposed to be 80% pure (and very nice electron
density, crystals diffracting to 1.7 A).
Remy Loris
Vrije Universiteit Brussel
Daniel Jin schreef:
Hi,
We would like to order two peptides for co-crystallizaiton. I
notice that there is a big price jump for peptide over 95% pure and
over 98% pure. Do you think 95% purity is good enough? Another
choice is to order crude peptides and purify by ourselves. Crude
peptide is much cheaper. But we will then have to put in time to do
multiple HPLC runs, reagent, cost for Mass Spec verification...
Does anyone have suggestion about which one will be more efficient?
Thanks.
Best,
Chen
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