Purification of crude peptides at low pressure can be done on 30RPC
(Pharmacia/Amersham/GE Healthcare) reverse phase resin in 0.1%TFA and
an acetonitrile gradient. It is easy if you are already equipped to do
protein preps. Lyophilize your peaks and bring up in DMSO.
Let me know if you need more details.
Tom Huxford.
Department of Chemistry & Biochemistry
San Diego State University
On Aug 27, 2008, at 10:04 AM, Daniel Jin wrote:
Hi,
We would like to order two peptides for co-crystallizaiton. I notice
that there is a big price jump for peptide over 95% pure and over 98%
pure. Do you think 95% purity is good enough? Another choice is to
order crude peptides and purify by ourselves. Crude peptide is much
cheaper. But we will then have to put in time to do multiple HPLC
runs, reagent, cost for Mass Spec verification... Does anyone have
suggestion about which one will be more efficient? Thanks.
Best,
Chen
