Dear Daniel,
We used peptides (up to 40 aa long) from BioSynthesis, Lewisville, Texas
(www.biosyn.com) at 90% purity with great success. The material is
guaranteed to be 90% (or whatever you ask) pure but in reality it is a
lot better. (I have no commerical interest here myself). In one case we
even crystallized a complex with peptide that was only supposed to be
80% pure (and very nice electron density, crystals diffracting to 1.7 A).
Remy Loris
Vrije Universiteit Brussel
Daniel Jin schreef:
Hi,
We would like to order two peptides for co-crystallizaiton. I notice
that there is a big price jump for peptide over 95% pure and over 98%
pure. Do you think 95% purity is good enough? Another choice is to
order crude peptides and purify by ourselves. Crude peptide is much
cheaper. But we will then have to put in time to do multiple HPLC
runs, reagent, cost for Mass Spec verification... Does anyone have
suggestion about which one will be more efficient? Thanks.
Best,
Chen
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