Meg wrote:
Dear All,
I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone give the
exact composition of how to prepare it. we prepare it using sodium acetate
and acetic acid combination. i am not able to arrive at the calculatation
correctly, so if anyone can explain me with the above buffer how to
calculate. and what sodium acetate [Anhydrous / trihydrate] and acetic acid
[glacial/ plain] to use.
thanks n regards
Meg goyal,
M.SC Biotechnology [Research]
Institute of science,
Fort
Mumbai, INDIA
If you want to get the pH exactly right, the only proper way to do this
is to make a solution of 25 mM acetic acid in water (leaving out about
20% of the water temporarily), then titrate with NaOH until you reach a
pH of 4.50. Then make up to the final volume with water and mix. You can
get the proportions approximately right by using the
Henderson-Hasselbalch equation, but in practice the pH may not come out
exactly right.
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