This is a job for the trusty Henderson-Hasselbach equation:
http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
On Jul 21, 2008, at 8:12 PM, Meg wrote:
Dear All,
I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone
give the
exact composition of how to prepare it. we prepare it using sodium
acetate
and acetic acid combination. i am not able to arrive at the
calculatation
correctly, so if anyone can explain me with the above buffer how to
calculate. and what sodium acetate [Anhydrous / trihydrate] and
acetic acid
[glacial/ plain] to use.
thanks n regards
Meg goyal,
M.SC Biotechnology [Research]
Institute of science,
Fort
Mumbai, INDIA
