Why not get an in-line, flow-cell LS detector for use with your
chromatography system? We once had a great system, set up with a good SEC
column, UV detection at three wavelengths on an akta, followed by SLS and
DLS, and refractive index detector. The data were beautiful, as the SEC made
the background very clean, and one could see easily the degree of
mono/polydispersity of the SEC peak(s). It was not, however, inexpensive.
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
*******************************************
----- Original Message -----
From: "Gregor Witte" <[EMAIL PROTECTED]>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Tuesday, July 08, 2008 10:27 AM
Subject: [ccp4bb] AW: [ccp4bb] DLS options?
Hmmm...
we had a demo of the Malvern Zetasizer instrument here, and to be honest: It
did not convince us at all (it is obviously built for non-biological
particle analysis)....
The Viscotek is also a plug&play device, every pc with a USB is suitable.
..in the end... Viscotek and Malvern are the same company now(!)
I guess the price for a DLS instrument is more or less independent of the
company.... aren't they all approx. 30-35k euros for a single-cuvette
system?
Gregor
-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von Roger
Rowlett
Gesendet: Dienstag, 8. Juli 2008 17:09
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] DLS options?
I'll second the recommendation for the Malvern Zetasizer. They are
rock-simple and interface with a computer through USB which makes future
computer upgrades relatively simple. The are however, not cheap--oops,
inexpensive.
Cheers,
--
------------------------------------------------------------------------
Roger S. Rowlett
Professor
Colgate University Presidential Scholar
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [EMAIL PROTECTED]
Andreas Förster wrote:
Hey Thomas,
also consider Malvern instruments. Their Zetasizers are really sweet
and work with volumes smaller than 15ul if you use the smallest cuvette.
http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm
The last DynaPro that I've used, half as old as the universe but
equipped with a 12ul cuvette, gave me really nice results also. Key is,
as you discovered, to keep the cuvette meticulously clean. I used
Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml
beaker, heat to 70C in the microwave with the cuvette inside, let sit
for a while, rinse with water and EtOH, and dry. Only touch with gloves
afterwards.
The protein sample must be spun down before the experiment. Half an
hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to
avoid bubbles when adding the sample to the cuvette.
Ah, nice data!
Hope that helps.
Andreas
Thomas Edwards wrote:
Dear BB,
Sorry for the off topic question:
I would like to buy a Dynamic Light Scattering system.
Could people suggest which they like the best and/or which is best value?
I have in the past used a Protein Solutions Dyna Pro with micro cuvette
(I would like a micro cuvette option). However, it was very sensitive to
dust and bubbles, and the cuvette collects dust.
I've never tried the one from Precision Detectors.
Any other options?
Thanks
Ed
______________
T.Edwards Ph.D.
Garstang 8.53d
Astbury Centre for Structural Molecular Biology
University of Leeds, Leeds, LS2 9JT
Telephone: 0113 343 3031
<http://www.bmb.leeds.ac.uk/staff/tae/>http://www.bmb.leeds.ac.uk/staff/tae/
<http://www.bmb.leeds.ac.uk/staff/tae/Research>
"The doubter is a true man of science; he doubts only himself and his
interpretations, but he believes in science". ~Claude Bernard
