Hey Thomas,
also consider Malvern instruments. Their Zetasizers are really sweet
and work with volumes smaller than 15ul if you use the smallest cuvette.
http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm
The last DynaPro that I've used, half as old as the universe but
equipped with a 12ul cuvette, gave me really nice results also. Key is,
as you discovered, to keep the cuvette meticulously clean. I used
Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml
beaker, heat to 70C in the microwave with the cuvette inside, let sit
for a while, rinse with water and EtOH, and dry. Only touch with gloves
afterwards.
The protein sample must be spun down before the experiment. Half an
hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to
avoid bubbles when adding the sample to the cuvette.
Ah, nice data!
Hope that helps.
Andreas
Thomas Edwards wrote:
Dear BB,
Sorry for the off topic question:
I would like to buy a Dynamic Light Scattering system.
Could people suggest which they like the best and/or which is best value?
I have in the past used a Protein Solutions Dyna Pro with micro cuvette
(I would like a micro cuvette option). However, it was very sensitive to
dust and bubbles, and the cuvette collects dust…
I’ve never tried the one from Precision Detectors.
Any other options?
Thanks
Ed
______________
T.Edwards Ph.D.
Garstang 8.53d
Astbury Centre for Structural Molecular Biology
University of Leeds, Leeds, LS2 9JT
Telephone: 0113 343 3031
<http://www.bmb.leeds.ac.uk/staff/tae/>http://www.bmb.leeds.ac.uk/staff/tae/
<http://www.bmb.leeds.ac.uk/staff/tae/Research>
"The doubter is a true man of science; he doubts only himself and his
interpretations, but he believes in science". ~Claude Bernard
