Hi Andrea, We have seen examples where it can make a dramatic difference, no expression even with codon plus strains, but really good expression with the daughter constructs as already pointed out. It is particularly true for parasite proteins where the percentage of AT is higher than bacteria. Also for mammalian kinases etc.
However, it can become a maximisation and not optimisation, hence can lead to trouble regarding toxicity or solubility. But there are expression system other than IPTG induced where one can fine tune these things. Regards, Partha On Feb 1, 2008 3:29 PM, Looney, Andrea Lynn (Andrea Hevrdeys) <[EMAIL PROTECTED]> wrote: > > > > Dear All, > > I am getting very little (not zero) expression of my protein. I am curious > to know if it is worthwhile to codon optimize my gene, which is ~1200bp, > for E.coli. Can you have "too much of a good thing"? Can codon optimizing > overwhelm the cell or in some way cause death or lowered expression? > > Thank you all, > > Andrea L. Hevrdeys > -- MRC National Institute for Medical Research Division of Molecular Structure The Ridgeway, NW7 1AA, UK Email: [EMAIL PROTECTED] Phone: + 44 208 816 2515
