Andrea,
Our experience with whole gene synthesis is that it often does not
increase levels of expression (or hurt), but it does allow one to more
efficiently make lots of "daughter" constructs from the original "parent"
construct. Most companies will charge X for the parent construct depending on
the length, and then will provide the daughter constructs at a much lower cost
since the DNA is being made anyway for the original. One can also design in
restriction sites to allow for more efficient design afterwards. It is likely
that by trying several different truncations (N and C termini, large and
small), you will increase your chances of success. The above being said, we
are occasionally surprised to get increases, so it is always worth a try.
Ray Stevens
________________________________
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Looney, Andrea
Lynn (Andrea Hevrdeys)
Sent: Friday, February 01, 2008 7:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Codon Optimized Expression
Dear All,
I am getting very little (not zero) expression of my protein. I am curious to
know if it is worthwhile to codon optimize my gene, which is ~1200bp, for
E.coli. Can you have "too much of a good thing"? Can codon optimizing
overwhelm the cell or in some way cause death or lowered expression?
Thank you all,
Andrea L. Hevrdeys
