Andrea,
Check your gene at http://nihserver.mbi.ucla.edu/RACC/ to see whether you have many rare codon clusters. With many rare codons, you may try those Codon-plus strains first. The effects of codon optimization is random, helpful to some genes but not to others. We had a few cases that are even detrimental to expression. Best, Chun Chun Luo, Ph.D. The Protein Expert Accelagen, Inc. 11585 Sorrento Valley Road, Suite 107 San Diego, CA 92121 TeL: 858-350-8085 ext 111 Fax: 858-350-8001 <mailto:[EMAIL PROTECTED]> [EMAIL PROTECTED] www.accelagen.com <http://www.accelagen.com/> _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Looney, Andrea Lynn (Andrea Hevrdeys) Sent: Friday, February 01, 2008 7:29 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Codon Optimized Expression Dear All, I am getting very little (not zero) expression of my protein. I am curious to know if it is worthwhile to codon optimize my gene, which is ~1200bp, for E.coli. Can you have "too much of a good thing"? Can codon optimizing overwhelm the cell or in some way cause death or lowered expression? Thank you all, Andrea L. Hevrdeys
