C-terminal 'clasp' domain of any bacterial gyrA or parC - very small, pretty tight dimers.
Artem > This is not strictly a crystallography question, but I imagine (and > hope) that some of you would be able to advise me. In short, I would > like to add to t7he N-terminal part of my construct a sequence that will > promote dimerization of the molecule. > > Background: I am working on a large protein that is built from multiple > domains. I am interested in the structure of some of the more C-terminal > domains. Obviously, I do not have well diffracting crystals at this > point (and hence this post). N-terminal to the domains of interest is a > dimerization domain. I would like to mimic the dimerization induced by > this domain by adding as short as possible a sequence. Moreover, I would > like to try both a parallel and an anti-parallel dimerization motif. > > Would you know of an appropriate short sequence (e.g. one that makes a > helix which dimerizes), or of a very small domain that I could add to my > construct? > > Thanks. > > Arnon >
