Search for e.g. Leucine zippers and you should find some references.
or e.g. Yamada et al. Protein Science 16(7) 1389-1397
Juergen
Arnon Lavie wrote:
This is not strictly a crystallography question, but I imagine (and
hope) that some of you would be able to advise me. In short, I would
like to add to the N-terminal part of my construct a sequence that
will promote dimerization of the molecule.
Background: I am working on a large protein that is built from
multiple domains. I am interested in the structure of some of the more
C-terminal domains. Obviously, I do not have well diffracting crystals
at this point (and hence this post). N-terminal to the domains of
interest is a dimerization domain. I would like to mimic the
dimerization induced by this domain by adding as short as possible a
sequence. Moreover, I would like to try both a parallel and an
anti-parallel dimerization motif.
Would you know of an appropriate short sequence (e.g. one that makes a
helix which dimerizes), or of a very small domain that I could add to
my construct?
Thanks.
Arnon
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
