You might look at this paper: http://www.ncbi.nlm.nih.gov/sites/
entrez?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12433100
They used DEAE and have given (in the supporting materials) a ton of
useful information.
Cynthia
On Sep 3, 2007, at 4:27 PM, James Fraser wrote:
I'm forwarding this to the listserve for someone in my lab who is
having trouble sending to the list. Replies to the list or directly
to Nat Echols ([EMAIL PROTECTED]) are greatly appreciated...
Not question about crystallography, but that's the ultimate goal:
Has anyone used a custom-made buffer for use with the PBE media
sold by
GE? I found a bottle of Pharmacia PBE 94 and would like to use it,
but
if I did I would use it frequently and on a relatively large scale, so
I'd rather not pay for the (expensive and proprietary) Polybuffer.
Alternately, has anyone done something similar with DEAE/CM media?
I've
seen references to this (although I suspect the resolution won't be as
good), but not very good descriptions.
thanks,
Nat
____________________
Cynthia Kinsland, Ph.D.
Cornell University
Protein Facility Director
607-255-8844
