Hi,
- I think someone already pointed out that you should try P6522.
- check that it isn't really a twinned P61 or P65 with 2 per asymmetric unit
- buy DNA with BrdU and some more with IdU, and/or grow your protein
in SeMet - at your resolution, the more "real" phase info the better!
Phoebe
At 06:30 PM 8/29/2007, you wrote:
Hello,
I am trying to solve a multi-protein DNA complex structure
from a 3.6 A native data set. The target structure is a dimer (95
aa in each monomer) in complex with DNA( 15 base pairs) plus a
second protein of 131 aa. The data has been scaled to P6(1)22 sp.
gr. and one target structure is expected to be in the asymmetric
unit that corresponds to 68% solvent content. 70 % of the target
structure is known in two parts(two different pdb structures
previously solved contributing 55% and 15% of the target
structure). I tried with molrep and phaser considering the first
part(55%) as the search model but it turned out to no good solution
which all clashes with symmetry related copies. If I assume the sp.
gr. is not the case what else I can try. Any suggestion is well appreciated.
Thanks...
Raja
---------------------------------------------------------------------------------------------------------------------------
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
fax 773 702 0439
http://bmb.bsd.uchicago.edu/index.html
http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
