Hello,
I am trying to solve a multi-protein DNA complex structure from a 3.6
A native data set. The target structure is a dimer (95 aa in each monomer) in
complex with DNA( 15 base pairs) plus a second protein of 131 aa. The data has
been scaled to P6(1)22 sp. gr. and one target structure is expected to be in
the asymmetric unit that corresponds to 68% solvent content. 70 % of the target
structure is known in two parts(two different pdb structures previously solved
contributing 55% and 15% of the target structure). I tried with molrep and
phaser considering the first part(55%) as the search model but it turned out to
no good solution which all clashes with symmetry related copies. If I assume
the sp. gr. is not the case what else I can try. Any suggestion is well
appreciated.
Thanks...
Raja
