Raja Dey wrote:
Hello, I am trying to solve a multi-protein DNA complex structure from a 3.6 A native data set. The target structure is a dimer (95 aa in each monomer) in complex with DNA( 15 base pairs) plus a second protein of 131 aa. The data has been scaled to P6(1)22 sp. gr. and one target structure is expected to be in the asymmetric unit that corresponds to 68% solvent content. 70 % of the target structure is known in two parts(two different pdb structures previously solved contributing 55% and 15% of the target structure). I tried with molrep and phaser considering the first part(55%) as the search model but it turned out to no good solution which all clashes with symmetry related copies. If I assume the sp. gr. is not the case what else I can try. Any suggestion is well appreciated. Thanks... Raja
Have you tried SG P6 5 22? Same systematic absences.. Eleanor
