Dear all, I have a structure at 1.6A. At the end of refinement the Rfactor and Rfree are quite high (23/29.6%). Here are some info:
Morphology of crystals: large but thin plates (150 x 400 x 20 microns)
Number of residues: 160 (2 x 80); 103 water molecules
Space group: C2
Unit Cell: 47.75 33.61 91.04 90.000 103.635 90.000
Data is a merge of a low resolution and high resolution sweep. The
correlation between the two data sets is:
DATA SETS NUMBER OF COMMON CORRELATION RATIO OF COMMON B-FACTOR
#i #j REFLECTIONS BETWEEN i,j INTENSITIES (i/j) BETWEEN i,j
1 2 4049 0.973 1.0002 0.0001
I include the data collection and refinement statistics in the attachment.
Can you please suggest to me possible reasons for the high Rfact and Rfree?
Thanks,
Elisabetta.
--
Elisabetta Sabini, Ph.D.
Research Assistant Professor
University of Illinois at Chicago
Department of Biochemistry and Molecular Genetics
Molecular Biology Research Building, Rm. 1108
900 South Ashland Avenue
Chicago, IL 60607
U.S.A.
Tel: (312) 996-6299
Fax: (312) 355-4535
E-mail: [EMAIL PROTECTED]
Arnonccp4.doc
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