Hi, How much salt do you have in your protein buffer? I would try to increase the salt concetration, during purification.
Hope it helps. Ana [EMAIL PROTECTED] wrote: > > Dear all, > > Sorry for the off-topic question. > > I am purifying a zinc finger transcription factor for crystallization. > The protein appeared as a single band on SDS-PAGE (MW 44KD) after NTA > chelating column, but its OD280/OD260 ratio is as high as 1.0. So I > doubt the protein is nucleic acid contaminated, probably because of > the zinc finger. I tried to remove the nucleic acid by Mono Q and > Superdex 75 pg, but failed. So could any one recommend some method to > remove the nucleic acid during protein crystallization, esp. zinc > finger protein? Any experience or references will be appreciated. > > Thanks a lot! > > Tiancen Hu > > Shanghai Institute of Materia Medica > > > > ------------------------------------------------------------------------ > 一起 来，150 万 人 同 时 在 玩 的 梦 幻 西 游 > <http://event.mail.163.com/chanel/xyq.htm?from=126_NO4>
