Dear all,
Sorry for the off-topic question.
I am purifying a zinc finger transcription factor for crystallization. The
protein appeared as a single band on SDS-PAGE (MW 44KD) after NTA chelating
column, but its OD280/OD260 ratio is as high as 1.0. So I doubt the protein is
nucleic acid contaminated, probably because of the zinc finger. I tried to
remove the nucleic acid by Mono Q and Superdex 75 pg, but failed. So could any
one recommend some method to remove the nucleic acid during protein
crystallization, esp. zinc finger protein? Any experience or references will be
appreciated.
Thanks a lot!
Tiancen Hu
Shanghai Institute of Materia Medica
